Sp1 binding site. Here we explore the molecular mechanisms underlying .

Sp1 binding site. We previously described a polymorphic Sp1 binding site in the COL1A1 gene that has been associated with osteoporosis in several populations. Significance Transcription factor binding to target DNA sequences is critical for regulating gene expression in response to environmental and developmental cues. Here, we use an inducible time-course ChIP-seq method to analyze SP1 binding dynamics at target sites throughout the human genome. There are Sp1 binding sites near the known HRE region in various genes, and Sp1 binding is required for the full activity of the HRE within gene-regulatory regions (Figure 1 A). The Sp1 transcription factor is defined as a ubiquitously expressed protein that contains three C2H2-type zinc fingers and binds to specific DNA sequences, primarily GC-boxes, to regulate transcription. Since the consensus binding site of transcription factor Sp1 contains a central CpG, we have investigated the binding of Sp1 factor to unmethylated and synthetically CpG-methylated DNA. The HIF-ARNT complex can occupy the enhancer region proximal or distal to the core promoter region of multiple genes, leading to increased transcriptional activities [3]. In contrast, other deletions or site-specific mutations re-veal an essential Sp1 site in the basal promoter and several dispersed upstream Sp1 sites that contribute to maximal reporter gene expression. In addition, Sp1 can interact with tissue-specific transcription factors to generate tissue-specific gene expression programs. Its activity is modulated by post-translational modifications and interactions with other proteins, including tumor suppressors and oncogenes. Aug 22, 1997 · In contrast, other deletions or site-specific mutations reveal an essential Sp1 site in the basal promoter and several dispersed upstream Sp1 sites that contribute to maximal reporter gene expression. Here we explore the molecular mechanisms underlying . Here, we use an inducible time-course ChIP-seq method to analyze SP1 binding dynamics at target sites throughout the Aug 1, 2015 · Sp1 (specificity protein 1) is a well-known member of a family of transcription factors that also includes Sp2, Sp3 and Sp4, which are implicated in an ample variety of essential biological processes and have been proven important in cell growth, differentiation, apoptosis and carcinogenesis. Sp1 activates the transcription of many cellular genes that contain putative CG-rich Sp-binding sites Multiple Sp1-binding elements are present in the MnSOD promoter (Fig. Sp1 is composed of several functional domains, such as the inhibitory domain (ID), two serine/threonine-rich domains, two glutamine-rich domains, three C2H2-type zinc finger DNA binding domains (ZFDBD), and a C-terminal D domain. "The Sp1 transcription factor gene (SP1) and the 1,25-dihydroxyvitamin D3 receptor gene (VDR) are colocalized on human chromosome arm 12q and rat chromosome 7". Pu. 2); removal of Sp1 sites in the promoter abrogates the transcription, even in the presence of NF-κB [38], suggesting the importance of this protein in the transcription processes. Apr 1, 2001 · Osteoporosis is a common disease with a strong genetic component. SP1 is a transcriptional activator protein that binds GC-box DNA sequences at many promoter and enhancer regions. 1 binding exposes the binding site for Sp1 in the chromatin, allowing it to bind and regulate transcription in a tissue-specific manner [97]. Nov 10, 2021 · Using a tamoxifen-inducible time-course ChIP-sequencing (ChIP-seq) approach, we show that the ubiquitous transcription factor SP1 has different binding dynamics at its target sites in the human genome. auo jzux mo7il uj 6mvw bor np9x hpesbu 5h9qe vi