Aggrecan neoepitope antibody. Validated Applications: WB, Flow, ICC/IF, IHC.
Aggrecan neoepitope antibody. Every product we sell is backed by Novus' 100% Guarantee. Validated Applications: WB, Flow, ICC/IF, IHC. If you have used this product, please submit your images and reviews to earn reward points. Anti-Aggrecan Neoepitope Antibody: Cited in 29 publications. All Novus Biologicals antibodies are covered by our 100% guarantee. Monoclonal antibodies have been prepared that react specifically with the neoepitopes present on proteoglycan degradation products generated from the proteolytic cleavage of aggrecan in the interglobular domain. Novus Biologicals Rabbit Aggrecan Neoepitope Antibody - BSA Free (NB100-74350) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Aggrecan Neoepitope (ACAN) is a proteoglycan belonging to aggrecan/versican proteoglycan family and is a major component of extracellular matrix of cartilagenous tissues. Western Blot Analysis: A representative lot detected Aggrecan neoepitope in human chondrocite cell lysate. 2), which detects chondroitin-4-sulfate stubs that remain attached to the core protein after chondroitinase ABC digestion. There are currently no images for Aggrecan Neoepitope Antibody (NB100-74350H). Our Aggrecan Neoepitope Antibodies can be used in a variety of model species: Bovine, Canine, Human, Mouse, Porcine, Rat. Validated Species: Human, Mouse, Rat + more. By Western blot, this antibody detects an ~64 kDa protein representing the neoepitope sequence (NITEGE) generated by aggrecanase-mediated cleavage at Glu373-Ala374 of aggrecan core proteins. Aggrecanase mediated cleavage results in ACAN fragments release from articular Each Aggrecan Neoepitope Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. ACAN proteoglycan is mainly consists of chondroitin sulfate, however, it also contains keratan sulfate chains, N/O-linked oligosaccharides. Novus Biologicals Rabbit Aggrecan Neoepitope Antibody - BSA Free (NB100-74350) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Degradation of IGD and accumulation of cleaved fragments was visualized via MEMCode reversible protein staining, followed by Western Blot using the BC-3 antibody for the specific detection of the aggrecan neoepitope. Aug 29, 2019 ยท In order to get a complete picture of aggrecanase activity, we recommend to use combinations of a neoepitope antibody and an antibody detecting total aggrecan fragments, such as the antibody 2B6 (Fig. Test in a species/application not listed above to receive a full credit towards a future purchase. In this chapter, we describe the production of two aggrecan neoepitope antibodies as examples: the monoclonal anti-FFGVG antibody (AF-28) and the polyclonal anti-DIPEN antisera. View Rabbit Polyclonal anti-Aggrecan Neoepitope Antibody [Alexa Fluor® 594] (NB100-74350AF594). Anti-Aggrecan (neoepitope) Antibody, clone BC-3 detects level of Aggrecan (neoepitope) & has been published & validated for use in Western Blotting & IHC. View Aggrecan Neoepitope Antibody [FITC] (NB100-74350F) validated in 6 species & 6 applications from Novus Biologicals. . Work with the C-terminal neoepitope antibodies has underscored the need to use a suite of neoepitope antibodies to fully describe aggrecanolysis in vitro. Recognizes the aggrecanase (ADAMTS-1, -4 & -5)-generated N-terminal neoepitope ARG after cleavage between amino acids EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan. f9 ksoszw rkjtm hor tls rofp rbyl6nx amaf ebx pawu